Welcome to the ChemBio Hub blog, a place for you to find out more about how we operate at a technical and collaborative level and how you can get involved. You can of course visit the ChemBio Hub website.

ChemBio Crunch - A simple tool to analyse and manipulate ChemBio assay data

posted 30 Sep 2014 by Andrew Stretton

The eventual goals of the ChemBio Hub project include enabling bench scientists to submit their biological activity data to public databases and to share it with specific people. When faced with this challenge an obvious research informatics solution would be to create a data repository and to then address the processes required to get data in there.

We decided the needs of the bench scientist had to come first in order to build a community around ChemBio Hub

Data is copied and pasted between different tools and Excel templates, calculated results may then be stored in a shared drive, database etc. Advantages of this approach include: - Familiarity and good documentation for the tools used - Low learning curve compared to programming - Flexibility, easy to adapt to changes in experimental setup

Issues that arise from this approach include: - Copy and paste errors leading to incorrect data - Management of template files in the group can be difficult - No consistent output data format -Duplication of effort between scientists and groups - Mixing of data and processing logic makes output hard to use

As we believe that there must be a better way - ChemBio Crunch was born

There are 4 simple steps to use ChemBio Crunch 1. Upload raw data files 2. Validate plate signals and remove outlier wells 3. Calculate IC50, hill slope and relevant errors 4. Export as XLSX, png or PPT

Results can then be compared using the publication-ready charting features of ChemBio Crunch

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